Single-molecule studies of genome processes
Our lab develops and applies innovative single-molecule approaches to elucidate the molecular mechanisms that drive genomic processes. To this end, we employ a combination of optical tweezers, fluorescence microscopy and nucleic acid engineering. A key focus is on understanding how changes in DNA structure regulate genome function. Towards this goal, we are developing single-molecule strategies to unravel how enzymes, such as topoisomerases, regulate DNA topology and how DNA supercoiling can alter DNA-protein interactions.
Selected publications
Unravelling the mechanisms of Type 1A topoisomerases using single-molecule approaches.
Spakman, D., Bakx, J.A.M., Biebricher, A.S., Peterman, E.J.G., Wuite, G.J.L., King, G.A.
Nucleic Acids Research (2021) 49, 5470–5492
Spakman, D., King, G.A., Peterman, E.J.G., Wuite, G.J.L.
Scientific Reports (2020) 10, 9903
King, G.A., Burla, F., Peterman, E.J.G., Wuite, G.J.L.
Proceedings of the National Academy of Sciences USA (2019) 116, 26534–26539