Proteomics programme

The proteomics programme uses changes in cellular proteomes to study parallel dynamic changes in cellular function, primarily in the area of cell signal transduction. New paradigms in cellular function suggest that many aspects of cellular function are controlled by rapid, stochastic, combinatorial processes which are linked to changes in cellular proteomes. We monitor and analyse the spatial and temporal properties of the molecular networks and fluxes involved in living cells by following the linked proteome changes.

New technologies developed in our laboratories and elsewhere, including gel and non-gel protein separations, parallel imaging processes, high throughput automatic protein identification, soft ionisation mass spectrometry and bioinformatics enable us to follow imprinting of the cellular proteome by functional processes. We are working on new technologies to increase sensitivity and proteome coverage. These include ultra-high sensitivity multi-photon-detection methods that allow linear quantitation of proteins upwards from zeptomoles and over ca. 1E+8 ranges of concentration. We identify proteins and characterize their phenotypic changes using high sensitivity MALDI-, ion-trap- and FTICR-mass spectrometry.

Programme leader
Dr Konstantinos Thalassinos



Institute of Structural and Molecular Biology, University of London
Last modifi
ed in 2006 by
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